Template strand and Coding strand – Class 12 | Chapter – 6 | Biology Short Notes Series PDF

Template strand and Coding strand: The term template strand refers to the sequence of DNA that is copied during the synthesis of mRNA. The opposite strand (that is, the strand with a base sequence directly corresponding to the mRNA sequence) is called the coding strand or the mRNA-like strand because the sequence corresponds to the codons that are translated into protein.

Although RNA polymerase must recognize sequences on the template strand, by convention we draw the DNA sequence and regulatory signals on the “mRNA-like” strand. (This makes it simpler to directly determine the sequence of the resulting RNA.) The following cartoon shows this concept for a hypothetical gene.

Difference Between Template Strand and Coding Strand

Template Strand Coding Strand
  • Serves as a template for the mRNA synthesis during transcription
  • Serves as a complementary strand of the template strand
  • Consists of a sequence that is complementary to the mRNA
  • Consists of a sequence that is identical to the mRNA, except that thymine in DNA is replaced by uracil in mRNA
  • Template strand runs from 3′ to 5′
  • The coding strand runs from 5′ to 3′


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By Team Learning Mantras