Nitrogen Cycle: Apart from carbon, hydrogen and oxygen, nitrogen is the most prevalent element in living organisms. Nitrogen is a constituent of amino acids, proteins, hormones, chlorophylls and many of the vitamins.
Nitrogen Cycle is a biogeochemical process through which nitrogen is converted into many forms, consecutively passing from the atmosphere to the soil to organism and back into the atmosphere.
It involves several processes such as nitrogen fixation, nitrification, denitrification, decay and putrefaction.
Nitrogen gas exists in both organic and inorganic forms. Organic nitrogen exists in living organisms, and they get passed through the food chain by the consumption of other living organisms.
Inorganic forms of nitrogen are found in abundance in the atmosphere. This nitrogen is made available to plants by symbiotic bacteria which can convert the inert nitrogen into a usable form – such as nitrites and nitrates.
Nitrogen undergoes various types of transformation to maintain a balance in the ecosystem. Furthermore, this process extends to various biomes, with the marine nitrogen cycle being one of the most complicated biogeochemical cycles.
The importance of the nitrogen cycle are as follows:
Process of the Nitrogen Cycle consists of the following steps – Nitrogen fixation, Nitrification, Assimilation, Ammonification and Denitrification. These processes take place in several stages and are explained below:
It is the initial step of the nitrogen cycle. Here, Atmospheric nitrogen (N2) which is primarily available in an inert form, is converted into the usable form -ammonia (NH3).
During the process of Nitrogen fixation, the inert form of nitrogen gas is deposited into soils from the atmosphere and surface waters, mainly through precipitation.
The entire process of Nitrogen fixation is completed by symbiotic bacteria, which are known as Diazotrophs. Azotobacter and Rhizobium also have a major role in this process. These bacteria consist of a nitrogenase enzyme, which has the capability to combine gaseous nitrogen with hydrogen to form ammonia.
Nitrogen fixation can occur either by atmospheric fixation- which involves lightening, or industrial fixation by manufacturing ammonia under high temperature and pressure conditions. This can also be fixed through man-made processes, primarily industrial processes that create ammonia and nitrogen-rich fertilisers.
In this process, the ammonia is converted into nitrate by the presence of bacteria in the soil. Nitrites are formed by the oxidation of ammonia with the help of Nitrosomonas bacteria species. Later, the produced nitrites are converted into nitrates by Nitrobacter. This conversion is very important as ammonia gas is toxic for plants.
The reaction involved in the process of Nitrification is as follows:
2NH3 + 3O2 → 2NO2– + 2H+ + 2H2O
2NO2– + O2 → 2NO3–
Primary producers – plants take in the nitrogen compounds from the soil with the help of their roots, which are available in the form of ammonia, nitrite ions, nitrate ions or ammonium ions and are used in the formation of the plant and animal proteins. This way, it enters the food web when the primary consumers eat the plants.
When plants or animals die, the nitrogen present in the organic matter is released back into the soil. The decomposers, namely bacteria or fungi present in the soil, convert the organic matter back into ammonium. This process of decomposition produces ammonia, which is further used for other biological processes.
Denitrification is the process in which the nitrogen compounds make their way back into the atmosphere by converting nitrate (NO3-) into gaseous nitrogen (N). This process of the nitrogen cycle is the final stage and occurs in the absence of oxygen. Denitrification is carried out by the denitrifying bacterial species- Clostridium and Pseudomonas, which will process nitrate to gain oxygen and gives out free nitrogen gas as a byproduct.
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