DNA Cloning: It refers to the process of creating multiple copies of a specific DNA sequence. This is achieved by inserting the target DNA sequence into a vector, such as a plasmid or bacteriophage, which is then introduced into a host organism, such as bacteria or yeast. The host organism replicates the vector along with its inserted DNA, resulting in multiple copies of the target DNA sequence.
The process of DNA Cloning can be broken down into several steps:
Purification of the target DNA: The purified target DNA can then be used for a variety of applications, such as sequencing, expression of the cloned gene, or further cloning experiments.
DNA Cloning has many important applications, including:
Studying evolution and genetics: It allows scientists to compare the DNA of different species, helping to understand the evolutionary relationships between different organisms and to study the genetic basis of traits and diseases.
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